Research Grants J. Klein (2013-2022)
Faculty Research Grant, University of Wisconsin ($14,600), Klein (PI), 07/01/2022-06/31/2023
Impacts of exercise-induced exosomes on muscle regeneration and immune cell regulation. In collaboration with the UW-La Crosse Department of Exercise and Sport Science, we are characterizing age-related changes in the miRNA content and regenerative potential of muscle exosomes released into the bloodstream following intense resistance training.
XSEDE Extreme Science and Engineering Environment ($78,000), Klein (PI), 05/01/2020-09/01/2020
Dynamic structural model of the SARS-CoV-2 main protease to guide drug design and repurposing. The project aims to provide a molecular-level structural prediction for why some SARS-CoV-2 protease inhibitors perform better than others, assist in the rational redesign of these inhibitors, and will set the stage for examining the impact of SARS-2 mutations on drug resistance.
WiSys Spark Grant ($10,000), Klein (PI), 02/01/2020-06/31/2020
Development of a novel epigenetic biomarker for food allergen tolerance during immunotherapy. We propose to (1) characterize genetic biomarkers for allergy immunotherapy, and (2) develop an allergy immunotherapy gene panel and predictive model that can be marketed to clinicians and patients.
Faculty Research Grant, University of Wisconsin ($16,750), Klein (PI), 07/01/2019-06/31/2020
Engineering resistance to oxidative stress in muscle. We are currently elucidating the pathway through which oxidative modification to the muscle protein calmodulin impairs myogenesis and will then use this knowledge to engineer resistance to oxidative stress in induced pluripotent stem cells for gene therapy.
XSEDE Extreme Science and Engineering Environment ($39,645), Klein (PI), 04/01/2017-03/31/18
Structural impact of calmodulin methionine oxidation. This funding provides supercomputing access and resources to carry out molecular dynamics simulations associated with my funded NIH award (below).
National Institutes of Health R15 ($329,169), Klein (PI), 09/15/2015-09/14/2019
Structural impact of methionine oxidation in muscle proteins. Our goal is to explain in molecular detail how age-related muscle degeneration is related to the oxidation of muscle proteins by biological oxidants, from atomic resolution molecular models to organismal aging.
Faculty Research Grant, University of Wisconsin ($12,000), Klein (PI), 07/01/2014-06/31/2015
Structural interaction of calmodulin-like protein with myosin-X. The goal of this project is to measure the structural interaction of calmodulin-like proteins with the unconventional myosins that they regulate.
Faculty Research Grant, University of Wisconsin ($12,500), Klein (PI), 07/01/2013-06/31/2014
Structural and functional impact of muscle protein oxidation. The goal of this project is to elucidate how site-specific oxidative modifications to muscle proteins, particularly the contractile protein myosin and the regulatory protein calmodulin, impact biological function and protein structural dynamics.